Discovery of the CRISPR system
EASL LiverTree™. Montoliu L. Apr 21, 2017; 173681
Topic: Genetic engineering
Dr. Lluis Montoliu
Dr. Lluis Montoliu

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To learn where the CRISPR system originally comes from, its origins as immune defense system in prokaryotes and its eventual convertion to the most efficient genome editing tool known to date.
In this presentation I review the origins of the CRISPR system. Initially discovered in bacteria, by several microbiologists, as a curious set of genomic repeated DNA sequences, were eventually found to be one of the immune systems in prokaryotes, adaptive, used to fight the intruding DNA elements, whether bacteriophages or plasmids. After years of studying the underlying mechanisms the elements constituting the CRISPR system were revealed and could be adapted for the simple, reliable and efficient genome editing of any cell or organism. The CRISPR system represents a disruptive technology, an irreverible change that has entirely transformed how we can edit genomes today. From the preparation of most adequate cellular and animal models to exploring novel and hopefully more successful approaches for somatic gene therapy, also for liver diseases.
Mojica FJ, Montoliu L. On the Origin of CRISPR-Cas Technology: From Prokaryotes to Mammals. Trends Microbiol. 2016 Oct;24(10):811-20. https://www.ncbi.nlm.nih.gov/pubmed/27401123
Josa S, Seruggia D, Fernández A, Montoliu L. Concepts and tools for gene editing. Reprod Fertil Dev. 2016 Jan;29(1):1-7.
https://www.ncbi.nlm.nih.gov/pubmed/28278788
Oliveros JC, Franch M, Tabas-Madrid D, San-León D, Montoliu L, Cubas P, Pazos F. Breaking-Cas-interactive design of guide RNAs for CRISPR-Cas experiments for ENSEMBL genomes. Nucleic Acids Res. 2016 Jul 8;44(W1):W267-71.
https://www.ncbi.nlm.nih.gov/pubmed/27166368
Seruggia D, Fernández A, Cantero M, Pelczar P, Montoliu L. Functional validation of mouse tyrosinase non-coding regulatory DNA elements by CRISPR-Cas9-mediated mutagenesis. Nucleic Acids Res. 2015 May 26;43(10):4855-67.
https://www.ncbi.nlm.nih.gov/pubmed/25897126
Harms DW, Quadros RM, Seruggia D, Ohtsuka M, Takahashi G, Montoliu L, Gurumurthy CB. Mouse Genome Editing Using the CRISPR/Cas System. Curr Protoc Hum Genet. 2014 Oct 1;83:15.7.1-27.
https://www.ncbi.nlm.nih.gov/pubmed/25271839
Seruggia D, Montoliu L. The new CRISPR-Cas system: RNA-guided genome engineering to efficiently produce any desired genetic alteration in animals. Transgenic Res. 2014 Oct;23(5):707-16.
https://www.ncbi.nlm.nih.gov/pubmed/25092533
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